Methods for producing recombinant coronavirus

US7279327B2 · kind B2 · utility

Assignee

• The University of North Carolina at Chapel Hill · US

Inventors

• Kristopher M. Curtis · Frederick, US
• Boyd Yount · Hillsborough, US
• Ralph Baric · Haw River, US

Key dates

• Filing date: Apr 19, 2002
• Grant date: Oct 9, 2007
• Expiry date: May 22, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A helper cell for producing an infectious, replication defective, coronavirus (or more generally nidovirus) particle cell comprises (a) a nidovirus permissive cell; (b) a nidovirus replicon RNA comprising the nidovirus packaging signal and a heterologous RNA sequence, wherein the replicon RNA further lacks a sequence encoding at least one nidovirus structural protein; and (c) at least one separate helper RNA encoding the at least one structural protein absent from the replicon RNA, the helper RNA(s) lacking the nidovirus packaging signal. The combined expression of the replicon RNA and the helper RNA in the nidovirus permissive cell produces an assembled nidovirus particle which comprises the heterologous RNA sequence, is able to infect a cell, and is unable to complete viral replication in the absence of the helper RNA due to the absence of the structural protein coding sequence in the packaged replicon. Compositions for use in making such helper cells, along with viral particles produced from such cells, compositions of such viral particles, and methods of making and using such viral particles, are also disclosed.