Rhodococcus nitrile hydratase

US7288402B2 · kind B2 · utility

Assignee

• Degussa AG · DE

Inventors

• Steffen Osswald · Rodenbach, DE
• Stefan Verseck · Hanau, DE
• Uta Deiting · Frankfurt, DE
• Christoph Weckbecker · Gelnhausen, DE
• Klaus Huthmacher · Gelnhausen, DE
• Michael Binder · Steinhagen, DE
• Maria-Regina Kula · Jülich, DE
• Konrad Odendahl

Key dates

• Filing date: Mar 18, 2005
• Grant date: Oct 30, 2007
• Expiry date: Mar 18, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.