Electrochemical detection of mismatch nucleic acids

US7291457B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Jeffrey Miller · New York, US
• Jun Huang · Lo Wu, CN
• Tza-Huei Wang · Timonium, US
• Chih-Ming Ho · Los Angeles, US
• Minghsun Liu · Los Angeles, US

Key dates

• Filing date: Jun 21, 2002
• Grant date: Nov 6, 2007
• Expiry date: Jun 21, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6827
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method and system for specifically detecting perfectly matched probe-target hybrids by annealing a hairpin-based probe to a nucleic acid target under conditions favoring the formation of perfectly matched targets over mismatched targets, and electrochemically detecting the annealed probe. Sensitivity of detection can be enhanced by catalyzed reporter deposition of probe signal prior to electrochemical detection. Specificity can be enhanced by including hairpin-based competitor probes in the annealing step. The detection assay is particularly applicable to typing single nucleotide polymorphisms by DNA sandwich hybridization.