Cloning pigs using non-quiescent differentiated donor cells or nuclei

US7291764B1 · kind B1 · utility

Assignee

• University of Massachusetts, a Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus, Office of Vice Chancellor for Research at Amherst · US

Inventors

• Steven Stice · Granby, US
• Jose Cibelli · Amherst, US
• James Robl · Granby, US
• Paul Golueke · Granby, US

Key dates

• Filing date: Sep 13, 1999
• Grant date: Nov 6, 2007
• Expiry date: Sep 13, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8778
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.