Process for producing nerve stem cells, motor neurons, and GABAergic neurons from embryonic stem cells
US7294510B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 3, 2001 |
| Grant date | Nov 13, 2007 |
| Priority date | — |
| Expiry date | Apr 29, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/32
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a method for producing motor neurons and GABAergic neurons characterized by including suspension-culturing embryonic stem cells in the presence or absence of a protein noggin to form embryoid bodies, selectively amplifying into neural stem cells from them by suspension culture in the presence of a fibroblast growth factor and a sonic hedgehog protein, and then differentiating the same. According to this method, at least motor neurons and GABAergic neurons can be systemically and efficiently produced from ES cells. Selective acquisition of neurons would be applicable to transplant therapy for amyotrophic lateral sclerosis, Huntington's chorea, Alzheimer's disease, etc.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.