Intermediates and enzymes of the non-mevalonate isoprenoid pathway

US7297509B2 · kind B2 · utility

Assignees

• Adelbert Bacher · DE
• Felix Rohdich · DE

Inventors

• Adelbert Bacher · Garching bei München, DE
• Felix Rohdich · Freising, DE
• Petra Adam · Neustadt an der Aisch, DE
• Sabine Amslinger · Idar-Oberstein, DE
• Wolfgang Eisenreich · Garching bei München, DE
• Stefan Hecht · Bad Aibling, DE

Key dates

• Filing date: Apr 10, 2002
• Grant date: Nov 20, 2007
• Expiry date: Jun 22, 2022

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/04
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The invention provides a protein in a form that is functional for the enzymatic conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate to 1-hydroxy-2-methyl-2-butenyl 4-diphosphate notably in its (E)-form of the non-mevalonate biosynthetic pathway to isoprenoids. The invention also provides a protein in a form that is functional for the enzymatic conversion of 1-hydroxy-2-methyl-2-butenyl 4-diphosphate, notably in its (E)-form, to isopentenyl diphosphate and/or dimethylallyl diphosphate. Further, screening methods for inhibitors of these proteins are provided. Further, 1-hydroxy-2-methyl-2-butenyl 4-diphosphate is provided and chemical and enzymatic methods of its preparation.