Cell lines that stably or transiently express a functional sweet (T1R2/T1R3) taste receptor
US7297543B2 · kind B2 · utility
60Cited by
3References
65Claims
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Key dates
| Filing date | Dec 2, 2003 |
| Grant date | Nov 20, 2007 |
| Priority date | — |
| Expiry date | Dec 2, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Cells are engineered to stably or transiently express T1R2 and T1R3 polypeptides, desirably in association with a G protein. The resultant heteromeric taste receptor is a sweet taste receptor and responds to sweet taste stimuli such as artificial and natural sweeteners, e.g., saccharin. The invention provides a preferred mammalian cell lines, e.g., HEK-293 cells that stably express T1R2/T1R3 and Gα15 under inducible conditions. These cells are useful in cell-based assays for identifying compounds that elicit or modulate sweet taste.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.