Process of purification of hCG and recombinant hCG purified by that method
US7297777B2 · kind B2 · utility
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7Claims
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Key dates
| Filing date | Jan 22, 2001 |
| Grant date | Nov 20, 2007 |
| Priority date | — |
| Expiry date | Aug 8, 2022 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P15/08
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for the purification of recombinant human Chorionic Gonadotropin (hCG) from a sample of crude recombinant hCG in the supernatant of CHO cells comprises the combined use of ion-exchange chromatography and reverse phase HPLC. The ion-exchange chromatography is performed twice and the final use of a size exclusion chromatography allows the purification from any residual traces of contaminants. The specific bioactivity of the highly purified hCG obtained form the process is particularly high, amounting to about 25,000 IU/mg.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.