Method of preparing polynucleotide fragments for use in shuffling, and shuffling of same
US7303897B2 · kind B2 · utility
0Cited by
5References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 17, 2003 |
| Grant date | Dec 4, 2007 |
| Priority date | — |
| Expiry date | Jun 10, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1027
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a fragmentation process that depends on mismatches between two strands of parental polynucleotides. One embodiment, comprises a method for preparing polynucleotide fragments of DNA comprising formation of heteroduplex molecules by hybridizing polynucleotides. The invention also provides a method and process of forming fragments which can be used with any shuffling process or combination of shuffling processes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.