Production of weakly active or inactive mutants of alkaline phosphatase and their expression in yeast

US7303903B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Rainer Mueller · Penzberg, DE
• Johann-Peter Thalhofer · Weilheim, DE
• Frank Geipel · Penzberg, DE
• Werner Hoelke · Penzberg, DE
• Thomas Kirschbaum · Munich, DE

Key dates

• Filing date: Mar 1, 2007
• Grant date: Dec 4, 2007
• Expiry date: Mar 1, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.