Retroviral vectors comprising a functional splice donor site and a functional splice acceptor site

US7303910B2 · kind B2 · utility

Assignee

• Oxford BioMedica (UK) Limited · GB

Inventors

• Christopher R. Bebbington · San Mateo, US
• Susan Kingsman · Felton, GB
• Mark Uden · Stevenage, GB
• Alan Kingsman · Shrewsbury, GB
• Kyriacos Mitrophanos · Shrewsbury, GB

Key dates

• Filing date: Apr 30, 2004
• Grant date: Dec 4, 2007
• Expiry date: May 12, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/44
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A retroviral vector is described. The retroviral vector comprises a functional splice donor site and a functional splice acceptor site; wherein the functional splice donor site and the functional splice acceptor site flank a first nucleotide sequence of interest (“NOI”); wherein the functional splice donor site is upstream of the functional splice acceptor site; wherein the retroviral vector is derived from a retroviral pro-vector; wherein the retroviral pro-vector comprises a first nucleotide sequence (“NS”) capable of yielding the functional splice donor site and a second NS capable of yielding the functional splice acceptor site; wherein the first NS is downstream of the second NS; such that the retroviral vector is formed as a result of reverse transcription of the retroviral pro-vector.