Total amino acid stabilization during cell-free protein synthesis
US7312049B2 · kind B2 · utility
25Cited by
3References
8Claims
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Key dates
| Filing date | Jun 5, 2006 |
| Grant date | Dec 25, 2007 |
| Priority date | — |
| Expiry date | Jun 5, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods are provided for the enhanced in vitro synthesis of protein molecules, by optimizing the metabolism of amino acids present in the reaction mix, preferably all amino acids in the reaction mixture. By performing synthesis with extracts from genetically modified microbial strains that are deficient in multiple amino acid metabolizing enzymes reduces the enzymatic activities responsible for catalyzing these reactions and improves the overall yield of synthesis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.