Insulin-secreting immortalized liver cell line modified by glucose sensitivity
US7312077B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Oct 2, 2002 |
| Grant date | Dec 25, 2007 |
| Priority date | — |
| Expiry date | Oct 2, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/04
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a cell line which can be substituted for β cells in human mature pancreatic islets and express insulin in a glucose-concentration dependent manner, and enables the easy obtainment of the number of cells which meets the demand. The present invention also provides a therapeutical cell preparation for treating diabetes. The cell lines of the present invention can be obtained by integrating both a nucleotide sequence encoding tamoxifen-induced Cre recombinase and a nucleotide sequence encoding insulin regulated by glucose-sensitive promoter into the chromosome in a human immortalized hepatic cell line FERM BP-7498 containing the TERT gene inserted in between a pair of LoxP sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.