Selection of target sites for antisense attack of RNA

US7313482B2 · kind B2 · utility

Assignee

• The Public Health Research Institute Of the City of New York, Inc. · US

Inventors

• Karl Drlica · New York, US
• Jian Wang · Paris, FR

Key dates

• Filing date: May 15, 2002
• Grant date: Dec 25, 2007
• Expiry date: Jun 30, 2022

Classification

• Technology area (CPC G): Physics
• CPC primary: G16B20/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Nucleic acid hybridization under steady-state conditions is described by a kinetic model in which the intermediate state is assumed to be locally single stranded. An expression was derived that relates nucleic acid secondary structure to the rate of oligonucleotide-RNA hybridization. The model allows the calculation of a rate factor that is proportional to the rate constant for hybridization between complementary nucleic acids and is generally applicable to any RNA molecule with potential utility for rapid identification of sites for antisense attack of mRNA.