Methods and materials for optimization of electronic transportation and hybridization reactions
US7314542B2 · kind B2 · utility
1Cited by
83References
19Claims
0Family size
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Key dates
| Filing date | Sep 22, 2005 |
| Grant date | Jan 1, 2008 |
| Priority date | — |
| Expiry date | Oct 25, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.