Apparatus and method for preparative scale purification of nucleic acids
US7314746B2 · kind B2 · utility
2Cited by
41References
7Claims
0Family size
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Key dates
| Filing date | Sep 12, 2003 |
| Grant date | Jan 1, 2008 |
| Priority date | — |
| Expiry date | Dec 20, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.