Constructs and methods for expression of recombinant HCV envelope proteins

US7314925B2 · kind B2 · utility

Assignee

• Innogenetics N.V. · BE

Inventors

• Erwin Sablon · Mechelen, BE
• Annie Van Broekhoven · Berchem, BE
• Fons Bosman · Opwijk, BE
• Erik Depla · Laarne, BE
• Geert Deschamps · Aalter, BE

Key dates

• Filing date: Apr 24, 2002
• Grant date: Jan 1, 2008
• Expiry date: Jun 1, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/24223
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The current invention relates to vectors and methods for efficient expression of HCV envelope proteins in eukaryotic cells. More particularly said vectors comprise the coding sequence for an avian lysozyme signal peptide or a functional equivalent thereof joined to a HCV envelope protein or a part thereof. Said avian lysozyme signal peptide is efficiently removed when the protein comprising said avian lysozyme signal peptide joined to a HCV envelope protein or a part thereof is expressed in a eukaryotic cell. Suitable eukaryotic cells include yeast cells such as Saccharomyces or Hansenula cells.