Method of determining adenovirus particle concentration
US7316898B2 · kind B2 · utility
2Cited by
5References
9Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Feb 14, 2003 |
| Grant date | Jan 8, 2008 |
| Priority date | — |
| Expiry date | May 13, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention discloses a simple, accurate and reproducible method of determining viral particle concentration, especially for any serotype of adenovirus. More specifically, an accurate absorptivity/extinction coefficient in units of viral particles per milliliter per absorbance unit per centimeter (vp/mL-AU-cm) for a sample virus, such as adenovirus, at 260 nanometers (nm) is disclosed, as well as an ultraviolet (UV) absorbance method to determine virus particle concentrations based on this established absorptivity/extinction coefficient and absorbances at 260 nm for purified virus preparations, such as adenovirus preparations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.