Overexpression, purification and characterization of a thermolabile phosphatase
US7319014B2 · kind B2 · utility
2Cited by
5References
1Claims
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Key dates
| Filing date | Feb 20, 2004 |
| Grant date | Jan 15, 2008 |
| Priority date | — |
| Expiry date | Aug 23, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.