Gene transfer into primate embryonic stem cells using VSV-G pseudotyped simian immunodeficiency virus vectors
US7323337B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | May 29, 2002 |
| Grant date | Jan 29, 2008 |
| Priority date | — |
| Expiry date | May 29, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/22
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Highly efficient gene transfer into primate-derived embryonic stem (ES) cells has successfully been achieved by using a simian immunodeficiency virus vector (SIV) pseudotyped with VSV-G protein, which is a surface glycoprotein of vesicular stomatitis virus (VSV) The present invention provides simian immunodeficiency virus vectors for gene transfer to primate ES cells. The method for gene transfer to primate ES cells using the vectors of the present invention is useful in, for example, research into embryology and disease, clinical applications, and experimental models for primates. The method is also useful in assaying and screening for genes and reagents able to enhance the specific differentiation of tissues or cells, and which are useful in preparing desired cells or tissues differentiated from ES cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.