Chromogenic enzyme substrates
US7323488B2 · kind B2 · utility
0Cited by
2References
11Claims
0Family size
Inventor
Key dates
| Filing date | Oct 24, 2002 |
| Grant date | Jan 29, 2008 |
| Priority date | — |
| Expiry date | Feb 4, 2024 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention provides novel chromogenic enzyme substrates which are indoxyl β-D-ribofuranosides. A process for their production is provided. Methods for detecting β-D-ribofuranosidase activity are given. The advantages of these novel compounds includes: detecting β-D-ribofuranosidase activity with high sensitivity and low substrate concentrations and use with other enzyme indicators in situations where a plurality of enzyme activities is to be visualized simultaneously e.g. for identifying bacteria growth on solid growth media. The synthesis and use of 5-bromo-4-chloro-3-indolyl-β-D-ribofuranoside is exemplified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.