Methods for isolating nucleic acids

US7329491B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Michael Kirchgesser · Taufkirchen, DE
• Frank Bergmann · Iffeldorf, DE
• Thomas Walter · Penzberg, DE
• Kurt Weindel · Schömberg, DE
• Ralf Zielenski · Benediktbeuern, DE
• Emad Sarofim · Hagendorn, CH

Key dates

• Filing date: Oct 12, 2004
• Grant date: Feb 12, 2008
• Expiry date: Aug 8, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1013
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; (b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.