Aptamers and methods for their in vitro selection and uses thereof
US7329742B2 · kind B2 · utility
12Cited by
8References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 3, 2004 |
| Grant date | Feb 12, 2008 |
| Priority date | — |
| Expiry date | Sep 23, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6811
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.