Multiphoton-excitation laser scanning microscope
US7329880B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 19, 2006 |
| Grant date | Feb 12, 2008 |
| Priority date | — |
| Expiry date | Apr 19, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/08
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A compact and inexpensive multiphoton-excitation laser scanning microscope for observing fluorescence by multiphoton excitation having a plurality of wavelengths by irradiating a specimen with ultrashort pulsed laser light having a plurality of wavelengths is provided. A multiphoton-excitation laser scanning microscope includes a laser light source that emits ultrashort pulsed laser light with a single wavelength, an optical fiber into which the ultrashort pulsed laser light from the laser light source is introduced and which broadens the spectrum of the ultrashort pulsed laser light, a laser scanning unit that scans the spectrally broadened ultrashort pulsed laser light emitted from the optical fiber, an objective optical system that focuses the scanned ultrashort pulsed laser light onto a specimen, optical detectors that detect fluorescence by multiphoton excitation emitted from the focal position of the ultrashort pulsed laser light in the specimen, and a dispersion-compensating optical system that compensates for the group velocity dispersion of the entire microscope that influences the pulse width of the ultrashort pulsed laser light.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.