Methods for detecting methylated nucleotides
US7332275B2 · kind B2 · utility
9Cited by
110References
13Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 15, 2002 |
| Grant date | Feb 19, 2008 |
| Priority date | — |
| Expiry date | Oct 15, 2022 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for detecting methylated nucleotides within a nucleic acid sample is provided. The method includes the steps of splitting a nucleic acid sample into separate reaction vessels; contacting nucleic acid in one reaction vessel with bisulfite; amplifying the nucleic acid in each reaction vessel; cleaving the nucleic acids in each reaction vessel to produce fragments thereof; and obtaining a mass spectrum of the resulting fragments from one reaction vessel and another mass spectrum of the resulting fragments from another reaction vessel.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.