Buffer solution for electroporation and a method comprising the use of the same

US7332332B2 · kind B2 · utility

Assignee

• amaxa AG · DE

Inventors

• Gudula Riemen · Langenfeld, DE
• Elke Lorbach · Cologne, DE
• Juliana Helfrich · Glauchau, DE
• Gregor Siebenkotten · Frechen, DE
• Herbert Muller-Hartmann
• Kirsten Rothmann
• Corinna Thiel · Cologne, DE
• Meike Weigel
• Heike Wessendorf
• Helmut Brosterbus · Witzenhausen, DE
• Michael Nix

Key dates

• Filing date: Apr 23, 2002
• Grant date: Feb 19, 2008
• Expiry date: Apr 20, 2023

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K48/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce biologically active molecules into cells using an electric current. The inventive buffer solution has a buffering capacity of at least 20 mmol−1×pH−1 and an ionic strength of at least 200 mmol×1−1 during a change to the pH value from pH 7 to pH 8 and at a temperature of 25° C. The use of a buffer solution of this type allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in the buffer solution.