Method for detecting analyte(s) using magnetic colloidal particles
US7332353B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 20, 2002 |
| Grant date | Feb 19, 2008 |
| Priority date | — |
| Expiry date | Jun 12, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5434
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention concerns a method for detecting and/or quantifying at least an analyte in a liquid medium, characterized in that it consists in using magnetic colloidal particles functionalized at the surface with at least a ligand specific of the analyte to be detected and/or assayed and in that it comprises: contacting said particles with said medium to be analyzed; applying a magnetic field to said medium, at an intensity sufficient to cause said magnetic particles to be structured in the form of chains; maintaining said magnetic field for a sufficient time interval to enable coupling or combination of the analyte concerned with at least two specific ligands present respectively on two neighbouring particles of a chain; cancelling the magnetic field, and determining the presence and/or absence of said analyte and, as the case may be, its concentration via the presence and/or the absence of said permanent chains of magnetic particles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.