Method for improving depth discrimination in optical reproduction systems
US7335866B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 28, 2003 |
| Grant date | Feb 26, 2008 |
| Priority date | — |
| Expiry date | Apr 25, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/361
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
The invention is directed to a method for improving the depth discrimination in optically imaging systems. It is applicable in particular in light microscopy for improving image quality when examining three-dimensionally extending objects. It is applicable in the method of structured illumination as described in WO 97/6509. For this purpose, influences due to variations in the brightness of the light source, positioning of the imaged periodic structure and bleaching of the object in fluorescence illumination are determined and taken into account in the calculation of the object structure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.