Recombinant methods for expressing a functional sweet (T1R2/T1R3) taste receptor
US7344859B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 3, 2003 |
| Grant date | Mar 18, 2008 |
| Priority date | — |
| Expiry date | Aug 29, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for expressing a functional heteromeric taste receptor that responds to sweet taste stimuli are provided. These methods comprise the co-expression of T1R2 and T1R3 nucleic acid sequences in a host cell that desirably further expresses a G protein that couples therewith, e.g., Gα15, Gα16 or gustducin. In preferred embodiments, the host cells will be mammalian cells or Xenopus oocytes. These nucleic acid sequences are expressed constitutively or under inducible conditions. In preferred embodiments the expression methods will use HEK-293 cells that also stably express Gα15. These methods give rise to heteromeric receptors and compositions containing that are useful in assays for identifying novel sweeteners and sweetness modulators.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.