DNA sequence analysis
US7351532B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 26, 2004 |
| Grant date | Apr 1, 2008 |
| Priority date | — |
| Expiry date | Jun 29, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
There is disclosed a method for determining the identity of one or more mutations or single nucleotide polymorphisms (SNPs) in a genome, comprising: a contacting a sample genome, under conditions which permit template dependant oligonucleotide ligation, with a plurality of different oligonucleotide molecules which comprise (i) a first set of oligonucleotides each comprising a sequence of nucleotides that is complementary to a region on said genome that includes a known SNP site and which oligonucleotides are complementary to said region other than at a base at or near the 5′ end of said oligonucleotides that is to be tested for complementarity to a base at the SNP site, each of said oligonucleotides comprising a unique label to identify both the base to be tested and the position of the SNP to be scored, (ii) a second set of oligonucleotides each comprising a sequence of nucleotides complementary to a region on said target genome for hybridisation with said target genome adjacent the 5′ end of an oligonucleotide of said first oligonucleotide set, and a surface capture moiety, a phosphate moiety being located at any of either the 5′ end of said first set of oligonucleotides or the 3…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.