Cell-free extracts and synthesis of active hydrogenase

US7351563B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• James R. Swartz · Menlo Park, US
• Marcus Emil Boyer · Palo Alto, US
• James Alan Stapleton · Los Altos, US
• Alfred M. Spormann · Stanford, US
• Chia-Wei Wang · Santa Clara, US

Key dates

• Filing date: Jun 10, 2005
• Grant date: Apr 1, 2008
• Expiry date: Apr 20, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/0067
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Enzymatically active hydrogenase is synthesized in a cell-free reaction. The hydrogenases are synthesized in a cell-free reaction comprising a cell extract derived from microbial strains expressing at least one hydrogenase accessory protein. In some embodiments, the extracts are produced under anerobic conditions.