QM-7 and QT-6 cells transfected with mutant cell surface expressed channel receptors and assays using the transfected cells
US7358057B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 9, 2003 |
| Grant date | Apr 15, 2008 |
| Priority date | — |
| Expiry date | Sep 10, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates e.g., to QM-7 or QT-6 cells comprising a heterologous mutant nicotinic α7 acetylcholine receptor and/or a nucleic acid encoding it, or a fragment or variant thereof. In a preferred embodiment, the mutant nicotinic α7 acetylcholine receptor subunit has a mutation in the M2 domain. QM-7 and QT-6 cells of the invention are useful for, e.g., assays such as high throughput assays that measure the influx of cations, such as Ca++ ions, into a cell. Such assays can be used, e.g., to identify agents that modulate the expression and/or activity of a mutant cell-surface-expressed channel receptor (e.g., the nicotinic α7 receptor), and which thus modulate, e.g., among other functions, processes involved in the central nervous system, such as learning and memory.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.