Production of 3-hydroxycarboxylic acid using nitrilase mutants
US7358071B2 · kind B2 · utility
20Cited by
6References
5Claims
0Family size
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Key dates
| Filing date | Nov 20, 2006 |
| Grant date | Apr 15, 2008 |
| Priority date | — |
| Expiry date | Nov 20, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/42
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidovorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.