Patent · US Expired

Method and device for electroporation of biological cells

US7358077B2 · kind B2 · utility

13Cited by

3References

6Claims

0Family size

Assignee

Eppendorf AG · DE

Inventors

Ulrich Zimmermann · Waldbrunn, DE
Vladimir Sukhorukov · Würzburg, DE
Kurt Lucas · Hamburg, DE

Key dates

Filing date	Jan 17, 2003
Grant date	Apr 15, 2008
Priority date	—
Expiry date	Jul 13, 2024

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N13/00
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Described are methods for electrical treatment of biological cells, in particular using electrical field pulses, involving the steps: arrangement of the cells (1) on apertures (2) of a solid planar carrier element (3) which divides a measuring chamber (10) into two compartments (11, 12); and temporary formation of an electrical treatment field which permeates the cells, wherein an alternating-current impedance measurement takes place on the carrier element (3), and from the result of the alternating-current impedance measurement, a degree of coverage of the carrier element and/or healing of the cells after electrical treatment are/is acquired. Also described are devices for implementing the methods.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.