Adaptive baseline algorithm for quantitative PCR
US7363168B2 · kind B2 · utility
5Cited by
2References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 1, 2002 |
| Grant date | Apr 22, 2008 |
| Priority date | — |
| Expiry date | Oct 24, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6851
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithm and methods and/systems implementing the algorithm.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.