Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy
US7364894B2 · kind B2 · utility
6Cited by
16References
22Claims
0Family size
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Key dates
| Filing date | Oct 15, 2003 |
| Grant date | Apr 29, 2008 |
| Priority date | — |
| Expiry date | Aug 26, 2024 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K48/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A prokaryotic recombinant host cell comprising a heterologous replication initiation protein that activates a conditional origin of replication and an extrachromosomal DNA molecule comprising a heterologous therapeutic gene and a conditional origin of replication whose functionality in the prokaryotic recombinant host cell requires a replication initiating protein which is foreign to the host cell is described. The host cell may comprise a pir gene having at least one mutation, which may occur in the pir gene copy number control region, the pir gene leucine zipper-like motif, or the pir gene DNA binding region.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.