Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7368239B2 · kind B2 · utility
5Cited by
5References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 26, 2004 |
| Grant date | May 6, 2008 |
| Priority date | — |
| Expiry date | Aug 5, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention provides methods and materials for conversion of cytosine to uracil. In some embodiments, a nucleic acid, such as gDNA, is reacted with bisulfite and a polyamine catalyst, such as a triamine or tetra-amine. Optionally, the bisulfite comprises magnesium bisulfite. In other embodiments, a nucleic acid is reacted with magnesium bisulfite, optionally in the presence of a polyamine catalyst and/or a quaternary amine catalyst. Also provided are kits that can be used to carry out methods of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.