Method for generating diversity
US7368259B2 · kind B2 · utility
3Cited by
3References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 11, 2003 |
| Grant date | May 6, 2008 |
| Priority date | — |
| Expiry date | Dec 11, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/56
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity; b) isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells; and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.