Selective genome amplification
US7368265B2 · kind B2 · utility
30Cited by
22References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 22, 2007 |
| Grant date | May 6, 2008 |
| Priority date | — |
| Expiry date | Jan 22, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods and compositions for amplifying selected polynucleotides, especially selected subsets of restriction fragments. Generally, methods of the invention are implemented by ligating adaptors containing at least one promoter sequence to such fragments under conditions that promote the formation of closed single stranded or double stranded structures, which are capable of serving as cyclical templates for transcription.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.