Expression of recombinant proteinase K from Tritirachium album in yeast

US7368274B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Rainer Mueller · Penzberg, DE
• Johann-Peter Thalhofer · Weilheim, DE
• Frank Geipel · Penzberg, DE
• Stephan Glaser · Seeshaupt, DE
• Werner Hoelke · Penzberg, DE
• Helmut Schoen · Penzberg, DE
• Thomas Kirschbaum · Munich, DE

Key dates

• Filing date: Feb 5, 2002
• Grant date: May 6, 2008
• Expiry date: Jun 14, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/58
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns a method for the expression of a gene coding for a soluble proteinase K in yeast e.g. in Pichia pastoris with subsequent secretion into the culture medium. In addition a method for purifying the heterologously expressed and secreted proteinase K is described.