Detection of biospecific interactions using amplified differential time domain spectroscopy signal
US7368280B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 22, 2003 |
| Grant date | May 6, 2008 |
| Priority date | — |
| Expiry date | Aug 1, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/3586
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detecting specific associations between a tethered molecule and an untethered target molecule. The method comprises (1) selecting a tethered molecule; (2) alternately impinging THz radiation onto the tethered molecule and onto a sample including the tethered and untethered target molecules; (3) detecting the radiation impinged on the tethered molecule to form a reference signal and the radiation impinged on the sample to form a sample signal; and (4) comparing the reference signal with the sample signal to generate a specimen signal indicative of an association between the selected tethered and target molecules. The method will detect whether a selected tethered molecule and the desired target exhibit any affinity or, in cases where the affinity is known, will detect the presence of the target molecule in a sample. Also provided is an apparatus for detecting specific associations between a tethered molecule and an untethered target molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.