Method and materials for bisulfite conversion of cytosine to uracil
US7371526B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 26, 2004 |
| Grant date | May 13, 2008 |
| Priority date | — |
| Expiry date | Nov 11, 2025 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention provides methods and materials for conversion of cytosine to uracil. In some embodiments, a nucleic acid, such as gDNA, is reacted with at least one bisulfite salt having the formula X+HSO3− or Y+2(HSO331)2; wherein X+ is ammonium ion, a tetraalkyl ammonium ion, or a group 1A ion other than sodium; and Y+2 is a group 2A ion or a group 7B ion; under conditions effective to convert at least one cytosine nucleobase to a uracil nucleobase. In some embodiments, X+ comprises at least one of lithium ion, potassium ion, ammonium ion, tetraalkylammonium ion, magnesium ion, manganese ion and calcium ion. In some embodiments, the reacting is performed optionally in the presence of a polyamine catalyst and/or a quaternary amine catalyst. Also provided are kits that can be used to carry out methods of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.