Multi-test analysis of real-time nucleic acid amplification
US7373253B2 · kind B2 · utility
44Cited by
5References
7Claims
0Family size
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Key dates
| Filing date | Feb 12, 2002 |
| Grant date | May 13, 2008 |
| Priority date | — |
| Expiry date | Mar 7, 2022 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L7/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and device are described for analyzing a sample for the presence of a nucleic acid wherein the sample is amplified, illustratively using PCR, in the presence of a fluorescent probe capable of providing a signal related to the quantity of nucleic acid present. A nucleic acid sample is amplified in the presence of the fluorescent entity, and the fluorescence intensity is measured at each of a plurality of amplification cycles. Scores are obtained from various tests performed on the fluorescence data, and the scores are used to determine whether the nucleic acid is present in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.