Method for isolating hepatitis C virus peptides

US7378234B2 · kind B2 · utility

Assignee

• Intercell AG · AT

Inventors

• Michael Buschle · Perchtoldsdorf, AT
• Andre Habel · Wien, AT
• Christoph Klade · Wien, AT
• Frank Mattner · Wien, AT
• Alexander Otava · Wien, AT
• Oresta Vytvytska · Wien, AT
• Wolfgang Zauner · Wien, AT
• Sandra Zinke · Wien, AT
• Helen Kirlappos · Wien, AT

Key dates

• Filing date: Aug 27, 2003
• Grant date: May 27, 2008
• Expiry date: Jun 29, 2024

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S930/223
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Described is a method for isolating Hepatitis C Virus peptides (HPs) which have a binding capacity to a MHC/HLA molecule or a complex comprising said HCV-peptide and said MHC/HLA molecule characterized by the following steps: —providing a pool of HCV-peptide, said pool containing HCV-peptides which bind to said MHC/HLA molecule and HCV-peptides which do not bind to said MHC/HLA molecule, —contacting said MHC/HLA molecule with said pool of HCV-peptides whereby a HCV-peptide which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said HCV-peptide and said MHC/HLA molecule is formed, —detecting and optionally separating said complex from the HCV-peptide which do not bind to said MHC/HLA molecule and optionally isolating and characterizing the HCV-peptide from said complex.