Method for determining the efficiency of nucleic acid amplifications

US7378241B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Gregor Sagner · Penzberg, DE
• Karim Tabiti · Pocking, DE
• Martin Gutekunst · Eberfing, DE
• Richie Soong · Tutzing, DE

Key dates

• Filing date: Mar 25, 2004
• Grant date: May 27, 2008
• Expiry date: Jun 1, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6851
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.