Fluorimetric determination of analytes by an intramolecular quencher-fluorophore conjugate

US7378255B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Carina Horn · Biblis, DE
• Hans-Peter Josel · Weilheim, DE
• Jurgen Spinke · Lorsch, DE
• Rupert Herrmann · Weilheim, DE
• Dieter Heindl · Munich, DE

Key dates

• Filing date: Jan 28, 2004
• Grant date: May 27, 2008
• Expiry date: Nov 28, 2024

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/904
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method and reagent for detecting an analyte by a redox reaction and a fluorimetric determination, is disclosed. The method comprises contacting a sample containing the analyte with a detection reagent which contains a compound of the general formula Q-F as a fluorimetric redox indicator, wherein Q is a quencher group and F is a fluorophore group.