Reagents and procedures for multi-label high-specificity labeling

US7381572B2 · kind B2 · utility

Assignee

• Rutgers, the State University of New Jersey · US

Inventors

• Richard H. Ebright · New Brunswick, US
• Yon W. Ebright · New Brunswick, US

Key dates

• Filing date: Oct 24, 2005
• Grant date: Jun 3, 2008
• Expiry date: Oct 24, 2025

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/805
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A detectable complex and methods for use thereof are provided herein. The detectable complex includes: at least one target material; a first peptide tag bound to the at least one target material; and a second peptide tag bound to the at least one target material. The complex further includes a first conjugate having a detectable group and two pendant phenylarsine moieties comprising a first tag binding group; wherein the first conjugate preferentially associates with the first peptide tag; and a second conjugate having a detectable group and two pendant phenylarsine moieties comprising a second tag binding group; wherein the second conjugate preferentially associates with the second peptide tag. The mean distance and/or mean angle between the pendant phenylarsine moieties in the first conjugate is different from the mean distance and/or mean angle between the pendant phenylarsine moieties in the second conjugate. Further provided herein are useful peptide tag combinations, target materials or sets of target materials including these peptide tag combinations, and nucleic acids or nucleic acid sets encoding these compositions.