Scintillation proximity assay method of measuring acetyl CoA carboxylase enzyme activity
US7384757B2 · kind B2 · utility
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1References
10Claims
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Key dates
| Filing date | Mar 30, 2005 |
| Grant date | Jun 10, 2008 |
| Priority date | — |
| Expiry date | Mar 30, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/988
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates generally to enzyme assays and more particularly to a rapid, sensitive, reliable and robust homogeneous assay for acetyl CoA carboxylase activity comprising a coupled enzyme assay in a scintillation proximity format suitable for high-throughput screening. In one aspect, the assay couples ACC and FAS and the product, palmitic acid is then detected by scintillation counting. The invention also relates to the identification of modulators of ACC activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.