Method of detecting β-D-ribofuranosidase activity
US7384763B2 · kind B2 · utility
1Cited by
1References
27Claims
0Family size
Inventor
Key dates
| Filing date | Oct 24, 2002 |
| Grant date | Jun 10, 2008 |
| Priority date | — |
| Expiry date | Jul 23, 2023 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention provides new methods for detecting enzyme activity using enzyme substrates comprising an enzyme cleavable portion and a chromogenic portion. The enzyme cleavable portion is a β-D-ribofuranosyl group and the chromogenic portion forms a detectable indicator following enzyme cleavage. In one aspect of this invention the chromogenic β-D-ribofuranoside substrate forms a colored substantially non-diffusable indicator. A second aspect of this invention provides novel chromogenic β-D-ribofuranosides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.