Cleavable linker for polynucleotide synthesis
US7385050B2 · kind B2 · utility
76Cited by
5References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 30, 2003 |
| Grant date | Jun 10, 2008 |
| Priority date | — |
| Expiry date | Nov 8, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/04
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Functionalized supports for polynucleotide synthesis are disclosed. The supports have linker moieties that are stable to conditions used in polynucleotide synthesis, but may be cleaved to release synthesized polynucleotides from the support. Methods of making the functionalized supports and methods of using are also disclosed. In particular embodiments of methods of making the functionalized supports, a solid support, on which an available reactive group is bound, is contacted with a reagent having the structure (I)Phos-Cgp-Trl-Cgp′Nucl (I)wherein the groups are defined as follows:
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.